GSM5696552: Zfp8 RNA-seq; Mus musculus; RNA-Seq - SRA

SRX13181821: GSM5696552: Zfp8 RNA-seq; Mus musculus; RNA-Seq
1 BGISEQ (BGISEQ-500) run: 49.5M spots, 4.9G bases, 2.8Gb downloads

External Id: GSM5696552_r1

Submitted by: Zhuang/ Shinohara, Immunology, Duke University

Study: Loss of Zfp335 triggers cGAS/STING-dependent apoptosis of post-B-selection pre-T cells [RNA-seq]

PRJNA782190 • SRP347039 • All experiments • All runs

show Abstracthide Abstract

The transcription factor Zfp335 is esstential for the survival of post-B-selection DN4 thymocytes. We utilized RNA-seq to profile the alterations to the transcriptional profiles of Zfp335-deficient DP thymocytes Overall design: DP thymocytes were FACS-sorted (Live Lin- CD4+ CD8+) from Zfp335-+/+ E8III-cre or Zfp335-fl/fl E8III-cre positive 7 week old female mice. Each sample was from one individual mouse. Following sorting, total RNA was isolated and libraries were prepared and sequenced at BGI genomics.

Sample: Zfp8 RNA-seq

SAMN23341766 • SRS11111205 • All experiments • All runs

Organism: Mus musculus

Library:

Name: GSM5696552

Instrument: BGISEQ-500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: DP thymocytes were isolated by FACS sorting. Cells were lysed in trizol and total RNA isolated using the Zymo Direct-zol RNA Microprep kit. Libraries were constructed at BGI genomics

Runs: 1 run, 49.5M spots, 4.9G bases, 2.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR16991460	49,470,376	4.9G	2.8Gb	2022-12-01

ID:: 18013863

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